Contact an Account ManagerQuantitative analysis of sulpiride in body fluids by HPLC with fluorescence detection
Summary
Type: HPLC
Application #: 103940
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence, excitation, 299nm, emission, 342nm
Conditions: eluent A: water - acetic acid (99:1)
eluent B: acetonitrile - acetic acid - water (50:1:49)
gradient 12 - 60% B in 10min
flow rate 1.2 - 1.6ml
pressure not to exceed 400psi
Authors: Alfredsson G
Source: J Chromatogr 164 (1979) 187 - 193
Details
Legend: Fig. 4. Chromatogram of a serum sample from a volunteer, sample taken 2h after
p.o. administration of 100mg sulpiride, drug concentration in serum 51ng/ml,
sample volume 10µl.